Biosequence searches are specialized searches that involve using sequence query as input for identification of identical or similar sequences. Biosequence searching comprises three basic steps – sequence searching, sequence analysis, and reporting. An expert sequence analyst is proficient is various databases (Commercial and non-fee search engines) and search tools (various algorithms and matrices). Human genome project has paved the wave for global genetic revolution which is evident from growing amount of genetic data being published and claimed with start of 21st century. Below are the primary reasons for conducting a biosequence search:
Our technical team has supported global clients in conducting sequence searches in various technology segments:
Therapeutics (Vectors and its components, Antibody chain sequences based on variable sequences, Biosimilars, Complementary Determining regions (CDRs), Enzymes/ Proteins, Peptides, small interfering RNAs (siRNAs); Diagnostics (Primers, Probes, Mutations, Variations, Markers, Antibodies, viral genome sequences); Allied Fields (Food, Industrial Biotechnology (biodiesel production), Forensic sciences)
Sequence analyst uses a combination of commercial databases (Databases hosted on STN – Registry, DGENE, USGENE, PCTGEN, GenomeQuest) and non-fee databases (Lens, NCBI) to retrieve potentially relevant dataset for analysis. Sequence analyst selects required algorithm/ program based on database recommendations (e.g. Blast, exact or sub-sequence/ family) while executing the search. We are specialized have proficiency conducted searches on modified sequences having mutations/ substitutions/ uncommon/ non-natural residues.
Note: We do not recommend any keyword based restrictions while conducting sequence based searches. Additionally, separate keyword based searches should be combined with sequence searching to comprehensively cover the technology domain.
